New research indicates that CBD and metformin could play a crucial role in managing behavioral difficulties in genetic conditions such as Fragile X and Phelan-McDermid syndromes. By correcting brain biochemistry and modifying behavior in mouse models, these substances offer hope for developing effective treatments for these disorders in humans.
New research highlights how
Early Intervention Studies with Metformin
In previous research,[2] Dr. Gantois and her colleagues have successfully used metformin to treat adult mice that were bred to model Fragile X syndrome. Metformin is the most widely used treatment for type 2 diabetes.
Now the researchers have tested metformin on newborn mice, also bred to model Fragile X syndrome. This research shows that metformin can correct a biochemical difference in the mouse’s brain that underlies the condition. The researchers also found that metformin stopped the young mice from developing impaired speech, which in mice manifests as very high-pitched squeaking, and repetitive behavior, which manifests as excessive grooming.
The research team has also used metformin with mice bred to model Phelan-McDermid syndrome and similarly found that they can reduce impaired speech and repetitive behavior, as well as improve the mice’s impaired learning and memory behavior.
Junghyun Choi, Laura Marsal-Garcia, Nahum Sonenberg and Ilse Gantois. Credit: FENS Forum / Ilse Gantois
Testing Cannabidiol’s Effects on Behavioral Disorders
Cannabidiol or CBD is the non-psychoactive component of cannabis. Dr. Gantois and her colleagues gave CBD to mice bred to model Fragile X syndrome from three weeks after birth. These mice are equivalent to young children. By the time the mice reached adulthood, their repetitive and social behaviors were the same as a control mouse. The researchers found similar improvements when treating mice bred to model Phelan-McDermid syndrome with CBD. The team are now studying how CBD interacts in the mouse brain to better understand why the drug affects behavior in this way.
Several other research groups in Canada and the USA are now running patient trials of metformin and CBD for Fragile X syndrome.
Dr. Gantois said: “This research and the outcome of the clinical trials could have a major impact on behavioral difficulties that people with Fragile X syndrome and Phelan-McDermid syndrome experience. The major strengths of these studies are that we are using approved drugs that can be taken throughout lifetime and have minimal side effects. Taken at an early age, these drugs could ultimately improve speech delay, social interaction, and repetitive behavior in these developmental conditions.
“Using mouse models makes it possible to look in detail at what is happening in the brain, define the underlying cause of these conditions, and study behavior and the effect of targeted drugs. Of course, mouse brains are not the same as human brains, however, we can often translate the mouse behavior we study to human behaviors.”
The researchers are also studying the effects of these drugs on mouse models of other autism spectrum disorder-related conditions to see if they could have broader use.
Professor Richard Roche is chair of the FENS Forum communication committee and Deputy Head of the Department of Psychology at Maynooth University, Maynooth, County Kildare, Ireland, and was not involved in the research. He said: “We need a lot more research to help us understand conditions like Fragile X and Phelan-McDermid and to find out how these developmental conditions can be treated to give children the best possible chance in life. Mouse models of these conditions give us a vital window into what is happening mechanistically inside the brain and allow us to study the possible benefits of new treatments.”
Notes
- “Repurposing of drugs for the treatment of Fragile X and Phelan McDermid syndromes”, by Ilse Gantois, Part of Scientific Symposia: S08 Dissecting mechanisms of disease in ASD models for developing and testing personalized treatments, 10:53 – 11:11 hrs CEST, Wednesday 26 June – Hall H, https://fens2024.abstractserver.com/program/#/details/presentations/71
- “Metformin ameliorates core deficits in a mouse model of fragile X syndrome” by Ilse Gantois, Arkady Khoutorsky, Jelena Popic, Argel Aguilar-Valles, Erika Freemantle, Ruifeng Cao, Vijendra Sharma, Tine Pooters, Anmol Nagpal, Agnieszka Skalecka, Vinh T Truong, Shane Wiebe, Isabelle A Groves, Seyed Mehdi Jafarnejad, Clément Chapat, Elizabeth A McCullagh, Karine Gamache, Karim Nader, Jean-Claude Lacaille, Christos G Gkogkas and Nahum Sonenberg, 15 May 2017, Nature Medicine.
DOI: 10.1038/nm.4335
Funding: This study is mainly funded by FRAXA foundation, Canadian Fragile X foundation, CIHR, Mental Health Commission of Canada, and Brain Canada. The Azrieli Foundation is funding the human FXS metformin clinical trials.
Discussion about this post