Mapping the Molecular Mayhem Caused by Huntington’s Disease

New Insights Into Neuronal Damage

Spearheaded by graduate student Giel Korsten from the group of Lukas Kapitein, the researchers discovered a major new way in which huntingtin aggregates damage cells when they examined neurons expressing the expanded version of the protein. The researchers found that many of the nerve cells had breaks in the membrane that separates the nucleus from the rest of the cell. This barrier, known as the nuclear envelope, protects and regulates the chromosomes inside the nucleus, allowing them to turn genes on and off as needed.

Kapitein and colleagues found that huntingtin aggregates inside the nucleus disrupt the protein meshwork that underlies and strengthens the nuclear envelope, making the membrane more likely to rupture. Using a specialized technique known as expansion microscopy to visualize the nuclear aggregates in high detail, the researchers saw that tiny fibrils stick out from the aggregates and poke through the meshwork underlying the nuclear envelope. The aggregates may also impair the cell’s ability to reseal the envelope once it breaks, the researchers found.

Implications of Nuclear Envelope Ruptures

“We have discovered that the aggregates associated with Huntington’s disease induce ruptures in the nuclear envelope that compromise its barrier function,” Kapitein says.

Over time, these disruptions in the nuclear envelope likely lead to damage of the nerve cell’s DNA and the misregulation of neuronal genes, cellular defects that have previously been linked to Huntington’s disease pathology.

Broader Impact on Neurodegenerative Diseases

Kapitein notes that several other neurogenerative diseases, including certain types of amyotrophic lateral sclerosis and frontotemporal dementia, are associated with the formation of protein aggregates inside the cell nucleus. “We speculate that nuclear aggregate–induced ruptures in the nuclear envelope represent a common contributor to neurodegeneration that initiates a cascade of deregulated processes culminating in neuronal death and neuroinflammation,” Kapitein says.

Reference: “Nuclear poly-glutamine aggregates rupture the nuclear envelope and hinder its repair” 16 August 2024, Journal of Cell Biology.
DOI: 10.1083/jcb.202307142