Detection Mode
Multimode microplate readers include different detection modes. From absorbance to fluorescence, researchers can choose the mode that best suits the application.
![Icon representing gain adjustment Icon representing gain adjustment](https://cdn.the-scientist.com/assets/articleNo/71350/iImg/50892/11-23-bmg-tipbox-03-l.png)
Gain Adjustment
The right gain setting enhances the signal-to-background ratio and measurement sensitivity. Adjust the gain to ensure proper recording of all data points in a run.
![Icons showing plate choices Icons showing plate choices](https://cdn.the-scientist.com/assets/articleNo/71350/iImg/50890/11-23-bmg-tipbox-01-l.png)
Plate Choice
Microplates come in different formats, colors, and well shapes. Select the right plate to increase signal-to-background ratios and improve measurement sensitivity.
![Icon representing flash number Icon representing flash number](https://cdn.the-scientist.com/assets/articleNo/71350/iImg/50893/11-23-bmg-tipbox-04-l.png)
Flash Number
Flash settings influence total measurement times and data quality. Select an appropriate flash number to reduce variability and ensure reliable and accurate data.
![Icon showing reading direction Icon showing reading direction](https://cdn.the-scientist.com/assets/articleNo/71350/iImg/50891/11-23-bmg-tipbox-02-l.png)
Reading Direction
Measuring from above or below the microplate well has different advantages. Choose the reading direction based on factors that affect target localization in the well.
![Icon representing focus adjustment Icon representing focus adjustment](https://cdn.the-scientist.com/assets/articleNo/71350/iImg/50894/11-23-bmg-tipbox-05-l.png)
Focus Adjustment
Tunable focal height ensures the best results. Adjust the microplate reader to detect the focal plane where the intensity of the sample signal is at its highest.