The original The original SARS-CoV-2 viral strain that emerged in early 2020 was able to latch on to sugars known as sialic acids, found on the surface of human cells, an ability that later strains did not retain.
This binding was found using a combination of magnetic resonance and extremely precise high-resolution imaging, conducted at the Rosalind Franklin Institute and University of Oxford, and published recently in the journal Science.
This unique ability in the early strain also raises the possibility that this is how the virus first transferred from animals to humans.
Comparison With Subsequent Variants
Subsequent variants of concern, such as Delta and Omicron, do not have this ability to grab sialic
Research Methods and Techniques
An international team led by scientists at the Rosalind Franklin Institute used magnetic resonance and complex imaging techniques to investigate further. Using a nuclear magnetic resonance (NMR) spectroscopy technique called saturation transfer difference, they developed a new, sophisticated analysis method to address the complex problem. They have called the technique universal saturation transfer analysis (uSTA).
Professor Ben Davis of the Rosalind Franklin Institute and University of Oxford, one of the paper’s senior authors, said: “Two of the ongoing mysteries of the coronavirus pandemic are the mechanisms behind viral transmission and the origins of the zoonotic leap.
“There is evidence that some influenza viruses can grab sialic acid on the surface of human host cells, and this has been seen in Middle Eastern Respiratory Syndrome (MERS), which is a coronavirus. Although SARS-CoV-2 variants of concern had not shown this mechanism, our research finds that the viral strain that emerged in early 2020 could use this as a way of getting into human cells.”
The Binding Mechanism and Evolution of the Virus
The binding mechanism is found on the end of the N-terminal domain, which is a part of the virus that evolves more rapidly. The domain has previously been implicated in sialic acid binding but until the Rosalind Franklin Institute team applied high-resolution precision imaging and analysis this was unproven.
As to why the virus has discarded the sugar-binding feature as it has evolved into new variants, Professor Davis hypothesizes that it may be necessary for the initial zoonotic leap into humans from animals but can then be hidden until it is required again – particularly if the feature is broadly detrimental to the virus’s mission of replication and infection within humans.
Correlation With Earlier Evidence and Implications
The finding correlates with evidence from the first wave in Italy. The Italian Genomics Consortium saw a correlation between the severity of
Reference: “Pathogen-sugar interactions revealed by universal saturation transfer analysis” by Charles J. Buchanan, Ben Gaunt, Peter J. Harrison, Yun Yang, Jiwei Liu, Aziz Khan, Andrew M. Giltrap, Audrey Le Bas, Philip N. Ward, Kapil Gupta, Maud Dumoux, Tiong Kit Tan, Lisa Schimaski, Sergio Daga, Nicola Picchiotti, Margherita Baldassarri, Elisa Benetti, Chiara Fallerini, Francesca Fava, Annarita Giliberti, Panagiotis I. Koukos, Matthew J. Davy, Abirami Lakshminarayanan, Xiaochao Xue, Georgios Papadakis, Lachlan P. Deimel, Virgínia Casablancas-Antràs, Timothy D. W. Claridge, Alexandre M. J. J. Bonvin, Quentin J. Sattentau, Simone Furini, Marco Gori, Jiandong Huo, Raymond J. Owens, Christiane Schaffitzel, Imre Berger, Alessandra Renieri, GEN-COVID Multicenter Study, James H. Naismith, Andrew J. Baldwin and Benjamin G. Davis, 23 June 2023, Science.
DOI: 10.1126/science.abm3125