Researchers have developed a guide for detecting rare B cells, crucial in understanding food allergies and immune responses.
Scientists at McMaster University have created an instruction manual that will help scientists across the globe find hard-to-detect B cells.
Led by PhD student Alyssa Phelps and Department of Medicine Assistant Professor Josh Koenig, researchers wanted to chart a path to finding these cells as part of their work in understanding food allergies. Their work will be published today (January 19) in the journal Nature Protocols.
Understanding B Cells
B cells are a type of immune cell that makes antibodies. These cells help fight conditions like cancer and infections but can also cause autoimmune diseases and allergies.
“One of the big problems with trying to study these B cells, the ones that make these antibodies that have all kinds of different and very important functions, is that they’re really, really, rare. It’s hard to find them. And so, you have to have very good tools that will help you study these things,” Koenig says.
To give an example of just how rare these cells can be, Koenig pointed to a peanut-specific B cell. It makes up less than 0.0001 percent of immune cells in human blood.
Advanced Methodology
The team adopted a method originally created by Justin Taylor, who now operates the Taylor Lab out of the University of Virginia. Taylor created a method using antigen tetramers to sensitively tag and enrich specific B cells so they can be detected.
Tetramers are made up of four antigen molecules, which in this case, can be customized by scientists. The customization is vast and can cover everything from peanuts to DOI: 10.1038/s41596-023-00930-8
The study was supported in part by a $10 million donation by the Schroeder Foundation to McMaster University. Funding was also provided from ALK Abello A/S, a pharmaceutical company based in Denmark.